Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry

The aim of this study was to evaluate the content and profile phenolic compounds (PCs) antioxidant properties field-grown leaves, in vitro leaves callus cultures blackberry ‘?a?anska Bestrna’ blueberry ‘Toro’. In shoots selected genotypes were grown either on original Murashige Skoog (MS) medium containing 1 mg/L BA, 0.1 IBA GA3 (‘?a?anska Bestrna’) or MS with macroelements reduced ½, 2 zeatin 0.2 IAA (‘Toro’). Callus induced from established BA 2,4-D half strength macroelements, NAA Total (TPC) flavonoid (TFC) highest whereas low TPC TFC values obtained both cultivars. A higher PCs compared determined by UHPLC-DAD MS/MS technique. Quercetin derivatives acids dominant berries, gallocatechin present a significant amount leaves. berries had specific PC profile, none detected except quercetin-3-O-glucoside quercetin-3-O-rutinoside. Blackberry showed best as estimated ferric reducing power (FRP), ABTS•+ DPPH• scavenging activity assays. exhibited three five times lower ten seventeen corresponding analyzed can be good source phenolics, respectively, for applications food pharmaceutical industries.